Amino Acid Analysis
Custom based analysis as well as method development
For free amino acid determination an appropriate extraction method will be applied. General sample pretreatment is included in the analysis cost.
Extensive or non-standard treatment will be discussed prior to acceptance of your order.
For total amino acid analysis, we perform:
- Acid hydrolysis for the stable amino acids
- Oxidation followed by acid hydrolysis for cyst(e)ine and methionine
- Alkaline hydrolysis for tryptophan. Di-peptide bound tryptophan can be also estimated by quantitative analysis of these di-peptides.
Analysis of amino acids is performed with HPLC-UV/FLU and/or Biochrom amino acid analysers using classical ion-exchange liquid chromatography with post-column Ninhydrin derivatisation and photometric detection. Li-Citrate as well as Na-Citrate buffer system elution is used to cover the total amino acid analysis field. Quantifications are performed with a suitable internal standard.
Our methods are based on EP 2.2.56, USP <1052> and EC directive L152/2009, Annex III. Samples originating from GMP production processes are analysed according to the relevant Ph. Eur. monographs or our own in-house developed and validated methods.
Do you want to inquire about possibilities or discuss any aspect of our service? Please feel free to contact us. We would like to get into touch with you.