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Amino acid analysis in Feed and Food Stuff


Since the discovery of mad cow disease and BSE problems at the end of the 90-ties, it is not allowed to use animal meal for the production of feed and food stuff.
However the vegetable material to produce feed and food stuff might be essential amino acid deficient:

Source

Wheat
Rice
Maize
Pulses

Limiting Amino Acid

Lysine
Lysine and threonine
Lysine and tryptophan
Methionine

Ansynth Service is specialized in classical ion-exchange amino acid analysis to detect deficiencies of essential amino acids in all kinds of vegetable material. For feed and food companies we routinely perform for this reason amino acid analysis. Then we estimate the purity of synthetic amino acids as lysine, threonine, methionine and tryptophan, to use them as supplements to vegetable material to produce nourishing feed and food.
Finally we check the total content of these originally deficient amino acids in the feed and food end product. 

Ansynth Service developed for these reasons special methods to estimate total amino acids in feed and food products e.g.:
-   The presence of added free methionine in feed and food products might give problems if the methionine in proteins
    is routinely oxidized to methionine sulphone, which is stable to acid hydrolysis. Methionine can then after acid
    hydrolysis be estimated as methionine sulphone.  A part of the added free methionine however will decompose
    by the oxidation reaction. So we adapted our method to avoid this problem

-   Tryptophan is acid labile. Therefore free tryptophan is estimated by HPLC.
    To measure total tryptophan we perform an alkaline hydrolysis

With our classical ion-exchange amino acid analysis with ninhydrin detection we follow AOAC and EU Commission Directives on the basis of our GMP/GcLP accreditation.

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