Peptide Synthesis Service

Ansynth Service B.V. provides a high quality peptide synthesis service at laboratory scale (research grade, 10 - 5000 mg) from standard peptide synthesis to complex peptide modifications mainly using Fmoc solid phase peptide synthesis. Custom peptides are built on a polystyrene resin stepwise from C- to N-terminus. Our manual peptide synthesis approach allows us to control and optimize the attachment of every amino acid and to estimate the coupling yield. After each step eventually remaining free amino functions of the growing peptide chain are terminated by means of an anhydride. Possible synthesis-related impurities in the crude peptide are mainly terminated sequences and almost no deletion sequences.

After detachment from the resin and removal of the side chain protecting groups the crude synthetic peptide can be purified. Dependent on the needs of the customer and the properties of the peptide a suitable target purity can be established. Most times, using preparative HPLC, crude peptides can be purified up to > 95% by HPLC.

For quality control of the synthesized peptides HPLC's with UV/Visible Light Detectors, and LC/MS methods are available.

In case a higher amount of synthetic peptide is demanded, it is preferred to first synthesize a small amount, e.g. 10 or 25 mg. This way information is obtained about peptide synthesis problems, yield, and properties of the peptide regarding the requested purity. Based on data of the pilot peptide synthesis a fair quotation for the intended amount can be proposed to the customer. The customer also has the opportunity to test the peptide before ordering a larger quantity.

In general Ansynth Service B.V. is synthesizing:

• peptides of different lengths in variable amounts
• sequences which drop out in automatic synthesis
• complex peptides as (poly-)cyclic peptides
• peptides with non-natural or modified amino acids
• peptides which could not be synthesized elsewhere, etc.

Please feel free to discuss with us your specific peptide synthesis needs

RGD PEPTIDES

In recent years, the interest for the application of (cyclic) RGD-peptides in tumour-targeting has increased. As a result of this we have developed methodologies for the attachment of labels. For example cyclo[DdFKRG] and cyclo[DdFKRA] are wanted peptides and also the lysine-derivatives (e.g. Ata) are popular. As we prepare custom made peptides other novel variants are also possible, please inquire.

Confidentiality Agreement

Before we can present a quotation, we need to have specific information and at least the peptide sequence. Principally we consider all given information as confidential. But in general we can agree with the preliminary need of a Confidentiality Agreement between the Customer and Ansynth Service B.V.

Delivery Remarks

We prefer to start the synthesis at the beginning of the week. The given delivery time is based on the production time of an average peptide, but:

• We cannot guarantee a successful synthesis and purification in advance
• Whether we are capable to synthesize and to purify the peptide can only be shown in practice
• Due to complications during the synthesis and/or purification the delivery time can deviate, in this case the customer will be informed as soon as possible
• If the synthesis is successful but not the total ordered amount, we can partially deliver. Partial deliveries can be invoiced
• Information will be given about the delivery time of the complete order
• Shipping cost for deliveries to foreign countries will be partly charged as indicated in the quotation.

Our products are for laboratory use only

Custom Peptide Synthesis Quotation and Order information

In our opinion all synthetic peptides are special, so Ansynth Service B.V. has no standard amino acid coupling prices.
We prefer to accept orders after a preliminary quotation. Upon request we are pleased to send a specified quotation.
The quoted price will depend on:

Synthetic aspects:

the length of the peptide and the amino acid composition
expected difficulty in coupling steps
the presence of special building blocks (unnatural amino acids, amino acid derivatives)
the need for intra- or inter-molecular bridges (e.g. (cyclic) disulfides, head-to-tail cyclisation)
other factors

Purification aspects:

required purity level e.g. crude, > 70%, > 80%, > 90% or > 95% on the basis of HPLC analysis
expected purity of the crude peptide after synthesis
expected physical properties of the peptide (solubility)
the presence of one or more Cysteines in the sequence, and the wish of a (poly-)-S-S-cyclic peptide, or an S-S-dimer, or especially a peptide with free SH-functional groups
the presence of other sensitive amino acids (e.g. methionine can be sensitive to oxidation, free N-terminal glutamine is prone to formation of pyroglutamate)
sensitivity for oxidation and/or spontaneous inter- and intra molecular chemical reactions or fragmentation reactions during the purification process and/or lyophilization cycle as a result of the amino acid sequence
etc

Quotation request can be send by e-mail (preferably) or by fax.
In general the customer's request will be quoted within 24 hours.
For preparing a quotation we need some information about the peptides to be synthesized:
sequence of the amino acids
N-terminus of the peptide (e.g. free or acetyl)
C-terminus of the peptide (e.g. amide or carboxyl)
amount (e.g. 10 mg, 25 mg, 100 mg)
purity (e.g. > 70%, > 80%, > 90%, > 95% by HPLC or without purification (crude))

• E-mail:
• Fax: +31165392087
• Post:

Ansynth Service B.V.
Department of Peptide Synthesis Service
Jan Vermeerlaan 202
NL - 4703 LE - ROOSENDAAL
The Netherlands

Other contact information:

• Phone: +31165392050

Necessary Ordering information:

Official Order form with Order number and the VAT-number (applicable for Europe)
Accurate description of the ordered peptide
Delivery address
Invoice address