Amino Acid Analysis Service

Within Ansynth Service B.V., 40 years Amino Acid Analysis experience is available with respect to the analysis of:

• Physiological, metabolic samples (plasma, urine, cerebrospinal fluid, amniotic fluid, seminal fluid, faeces, tissue extracts and homogenates, hair, etc.)
• Fruit juices, soft drinks, beer, wort, dairy food products, milk products or many other food stuffs
• Feed , fodder and silage
• Leaves, petals and seeds
• Protein or peptide based (pharmaceutical) products
• Synthetic peptides

Analysis of amino acids are performed with Biochrom amino acid analysers using classical ion-exchange liquid chromatography with post-column Ninhydrin derivatisation and photometric detection. Li-Citrate as well as Na-Citrate buffer system elution is used to cover the total Amino Acid Analysis yield.

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Samples originating from GMP production processes are analysed according to the relevant Ph. Eur. monographs.

Ansynth Service B.V. is licensed as a contract laboratory for quality control testing, and is considered to comply with the principles and guidelines of GMP/GcLP.

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Free and Total (bound) amino acids can be analysed. For total amino acids analysis, we perform:

    * Acid hydrolysis for most of the amino acids
    * Oxidation followed by acid hydrolysis for cyst(e)ine and methionine
    * Alkaline hydrolysis for tryptophan. Tryptophan is estimated by reversed phase HPLC

Example of physiological standards Apart from testing sample amino acids against a standard solution containing 39 amino acids, we also perform custom based analysis and method development.
Therefore we are able to analyse special amino compounds as:

Albizziin	Hydroxylysine
γ-Aminobutyric acid (GABA)	Hydroxyproline
δ-Amino-levulinic acid	5-Hydroxytryptophan
Cysteic acid	Lysinoalanine
Cysteine and Cystine	Methionine Sulfone
Cysteinyl-Homocysteine	Methionine Sulfoxide
Desmosine and Isodesmosine	S-Methylcysteine
Diaminobutyric acid (DAB)	α-Naphtylalanine
Diaminopimelic acid	Ornithine
Furosine	Pipecolic acid
Galactosamine and Glucosamine	Saccharopine
Glutathione (oxidized and reduced)	S-Sulfocysteine
Homocitrulline	Taurine and Hypotaurine
Homocystine and Homocysteine	Threoninol

Ansynth Service B.V. can also estimate the dipeptide bound tryptophan by quantitative analysis of these dipeptides.

Major applications of amino acid analysis
Quality control and identification of raw materials, intermediates and end-products
Registration of pharmaceuticals
Monitoring cell-culture or fermentation processes
Process control
Research and Development
Optimizing the composition of food, feed and dietary products
Compliance with nutritional regulations
Calibration of (FT)NIR systems

To Order and to Send samples:

• Pack the samples carefully to avoid losses, mixing of samples or fluid leakages
• Name the samples carefully and readable
• Cool the samples if necessary with ice or dry ice
• Please, use our order form related to our price list

Send the samples to:

Ansynth Service B.V.

attn. Mr. J. van Gool
Jan Vermeerlaan 204
NL - 4703 LE - ROOSENDAAL
The Netherlands

Phone: +31 165 536897
Fax: +31 165 537686
E-mail:

Analysis results will be presented by means of a Certificate of Analysis (CoA), Where applicable the CoA will be in a format and layout of your choice. CoA's will be sent by regular Postal Service, E-mail or Fax, usually within 2 - 5, or maximal 10 working days after receipt of the samples.