AMINO ACID ANALYSIS SERVICE
Within Ansynth Service B.V., 40 years Amino Acid Analysis experience is available
with respect to the analysis of:
- Physiological, metabolic samples (plasma, urine, cerebrospinal fluid, amniotic fluid, seminal fluid, faeces, tissue extracts and homogenates, hair, etc.)
- Fruit juices, soft drinks, beer, wort, dairy food products, milk products or many other food stuffs
- Feed , fodder and silage
- Leaves, petals and seeds
- Protein or peptide based (pharmaceutical) products
- Synthetic peptides
Analyses are performed with Biochrom Amino Acid Analysers using classical ion-exchange liquid chromatography with post-column Ninhydrin derivatisation and photometric detection. Li-Citrate as well as Na-Citrate buffer system elution is used to cover the total Amino Acid Analysis field.
Samples originating from GMP production processes are analysed according to the relevant Ph. Eur. monographs.
Ansynth Service B.V. is licensed as a contract laboratory for quality control testing, and is considered to comply with the principles and guidelines of GMP/GcLP.
Free and Total (bound) amino acids can be analysed. For Total Amino Acid Analysis, we perform:
- Acid hydrolysis for most of the amino acids
- Oxidation followed by acid hydrolysis for cyst(e)ine and methionine
- Alkaline hydrolysis for tryptophan. Tryptophan is estimated by reversed phase HPLC
Example of Physiological Standards
Apart from testing sample amino acids against a standard solution containing 39 amino acids, we also perform custom based analysis and method development. Therefore we are able to analyse special amino compounds as:
| Albiziin | Hydroxylysine |
| γ-Aminobutyric acid (GABA) | Hydroxyproline |
| δ-Amino-levulinic acid | Hydroxytryptophan |
| Cysteic acid | Lysinoalanine |
| Cysteine and Cystine | Methionine Sulfone |
| Cysteinyl-Homocysteine | Methionine Sulfoxide |
| Desmosine and Isodesmosine | S-Methylcysteine |
| Diaminobutyric acid (DAB) | α-Naphtylalanine |
| Diaminopimellic acid | Ornithine |
| Furosine | Pipecolic acid |
| Galactosamine and Glucosamine | Saccharopine |
| Glutathione (oxidized and reduced) | S-Sulfocysteine |
| Homocitrulline | Taurine and Hypotaurine |
| Homocystine and Homocysteine | Threoninol |
Ansynth Service B.V. can also estimate the dipeptide bound tryptophan by quantitative analysis of these dipeptides.
Major applications of amino acid analysis
| Quality control and identification of raw materials, intermediates and end-products |
| Registration of pharmaceuticals |
| Monitoring cell-culture or fermentation processes |
| Process control |
| Research and Development |
| Optimizing the composition of food, feed and dietary products |
| Compliance with nutritional regulations |
| Calibration of (FT)NIR systems |
To Order and to Send samples:
- Pack the samples carefully to avoid losses, mixing of samples or fluid leakages
- Name the samples carefully and readable
- Cool the samples if necessary with ice or dry ice
- Please, use our Orderform related to our Pricelist
- Send the samples to:
Ansynth Service B.V.
attn. Mr. J. Van Gool
Jan Vermeerlaan 204
NL - 4703 LE - ROOSENDAAL
The Netherlands
Phone: +31 165 536897
Fax: +31 165 537686
E-mail: analysis@ansynth.com
Analysis results will be presented by means of a Certificate of Analysis (CoA), Where applicable the CoA will be in a format and layout of your choice. CoA's will be sent by regular Postal Service, E-mail or Fax, usually within 2 - 5, or maximal 10 working days after receipt of the samples.
